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Alternative name
Proliferating cell nuclear antigen，Cyclin antibody
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Catalog
A006215
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat,Zebrafish
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Applications Tested/SuitableELISA,IF,IP,WB,IHC,FC
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PurificationImmunogen affinity purified
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Immunogenproliferating cell nuclear antigen
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IHCImmunohistochemistry of paraffin-embedded human stomach cancer using A006215(PCNA antibody) at dilution of 1:50
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Western BlotA431 cells were subjected to SDS PAGE followed by western blot with A006215(PCNA antibody) at dilution of 1:1000
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Recommended dilutionWB:1:1000-1:10000
IP:1:500-1:5000
IHC:1:200-1:1000
IF:1:20-1:200
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Product Description specificalProliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA.
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Gene namePCNA
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Uniprot IDP12004
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Calculated M.W.38kDa
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Observed M.W.36-38 kDa
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