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Alternative name
CPK, DKFZp686L193, PI 3 Kinase Class 2A, PI 3-kinase C2alpha, PI3 K C2(ALPHA), PI3 K C2A, PI3K C2 alpha, PIK3C2A antibody
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Catalog
A006449
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogenphosphoinositide-3-kinase, class 2, alpha polypeptide
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IHCImmunohistochemistry of paraffin-embedded human heart using A006449(PIK3C2A antibody) at dilution of 1:50
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Western BlotPC-3 cells were subjected to SDS PAGE followed by western blot with A006449(PIK3C2A antibody) at dilution of 1:1500
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalGenerates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin- mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin- independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function.
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Gene namePIK3C2A
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Uniprot IDO00443
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Observed M.W.191 kDa
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