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Alternative name
ATP5F1, ATPase subunit b, PIG47 antibody
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Catalog
A000707
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF
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PurificationImmunogen affinity purified
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ImmunogenATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
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IHCImmunohistochemistry of paraffin-embedded human liver tissue slide using A000707( ATP5F1 Antibody) at dilution of 1:200
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Western Blotmouse liver tissue were subjected to SDS PAGE followed by western blot with A000707(ATP5F1 antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
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Gene nameATP5F1
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Uniprot IDP24539
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Observed M.W.25 kDa
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