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Alternative name
ATP5A, ATPM antibody
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Catalog
A000711
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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ImmunogenATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
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IHCImmunohistochemistry of paraffin-embedded human osteosarcoma using A000711(ATP5J antibody) at dilution of 1:100
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Western Blothuman brain tissue were subjected to SDS PAGE followed by western blot with A000711(ATP5J antibody) at dilution of 1:800
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
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Gene nameATP5J
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Uniprot IDP18859
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Observed M.W.9 kDa
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