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Alternative name
SIPL1 antibody
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Catalog
A007838
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IP,IHC
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PurificationImmunogen affinity purified
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ImmunogenSHANK-associated RH domain interactor
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IHCImmunohistochemistry of paraffin-embedded human lymphoma tissue slide using A007838( SHARPIN Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western BlotRaji cells were subjected to SDS PAGE followed by western blot with A007838(SHARPIN antibody) at dilution of 1:500
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalComponent of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
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Gene nameSHARPIN
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Uniprot IDQ9H0F6
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Observed M.W.40 kDa
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