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Alternative name
TNK2, ACK1, ACK, FLJ45547, ACK 1 antibody
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Catalog
A000086
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogentyrosine kinase, non-receptor, 2
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IHCImmunohistochemistry of paraffin-embedded human breast cancer using A000086(ACK1 antibody) at dilution of 1:50
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Western BlotSH-SY5Y cells were subjected to SDS PAGE followed by western blot with A000086(ACK1 antibody) at dilution of 1:500
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Recommended dilutionWB :1:200-1:2000;IHC:1:20-1:200
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Product Description specificalTNK2, also named as ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.
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Gene nameTNK2
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locationwidely
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Uniprot IDQ07912
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Calculated M.W.115kDa,observed 70kDa
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Observed M.W.70 kDa
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