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Alternative name
EFP,RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147 antibody
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Catalog
A008976
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IF,IP
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PurificationImmunogen affinity purified
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Immunogentripartite motif-containing 25
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Western BlotMCF7 cells were subjected to SDS PAGE followed by western blot with A008976(TRIM25 antibody) at dilution of 1:500
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Recommended dilutionWB:1:200-1:2000;IP:1:200-1:2000;IF:1:20-1:200
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Product Description specificalFunctions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
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Gene nameTRIM25
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Uniprot IDQ14258
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Calculated M.W.71kDa
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Observed M.W.71 kDa
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