-
Alternative name
Ret finger protein, RFP, RING finger protein 76, RNF76, TRIM27, tripartite motif containing 27, Zinc finger protein RFP antibody
-
Catalog
A008977
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,WB,IHC,IP
-
PurificationImmunogen affinity purified
-
Immunogentripartite motif-containing 27
-
IHCImmunohistochemistry of paraffin-embedded human lung cancer using A008977(TRIM27 antibody) at dilution of 1:100
-
Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A008977(TRIM27 antibody) at dilution of 1:1000
-
Recommended dilutionWB:1:500-1:2000;IP:1:500-1:5000;IHC:1:20-1:200;IF:1:20-1:200
-
Product Description specificalE3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T- cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double- stranded DNA.
-
Gene nameTRIM27
-
Uniprot IDP14373
-
Calculated M.W.58kDa
-
Observed M.W.58 kDa
-