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Alternative name
C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I antibody
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Catalog
A009159
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF,FC
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PurificationImmunogen affinity purified
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Immunogenubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
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IHCImmunohistochemistry of paraffin-embedded human lung cancer using A009159(UBE2I-Specific antibody) at dilution of 1:50
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A009159(UBE2I-Specific antibody) at dilution of 1:400
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Recommended dilutionWB : 1:500-1:5000;IHC:1:20-1:200；IF:1:10-1:100
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Product Description specificalUBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).
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Gene nameUBE2I
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locationCytoplasm,Nucleus
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Uniprot IDP63279
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Calculated M.W.18kDa
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Observed M.W.18kd
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