-
Alternative name
HHR6A, HR6A, RAD6 homolog A, RAD6A, UBC2, UBE2A, Ubiquitin carrier protein A, Ubiquitin protein ligase A antibody
-
Catalog
A009163
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,WB,IHC
-
PurificationImmunogen affinity purified
-
Immunogenubiquitin-conjugating enzyme E2A (RAD6 homolog)
-
IHCImmunohistochemistry of paraffin-embedded human lymphoma using A009163(UBE2A antibody) at dilution of 1:50
-
Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A009163(UBE2A antibody) at dilution of 1:600
-
Recommended dilutionWB : 1:200-1:2000;IHC:1:20-1:200
-
Product Description specificalAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
-
Gene nameUBE2A
-
locationCytoplasm,uclear chromatin
-
Uniprot IDP49459
-
Calculated M.W.17kDa
-
Observed M.W.17 kDa
-