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Alternative name
E2-230K, FLJ12878, KIAA1734, UBE2O, Ubiquitin carrier protein O, Ubiquitin protein ligase O antibody
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Catalog
A009181
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB
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PurificationImmunogen affinity purified
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Immunogenubiquitin-conjugating enzyme E2O
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A009181(UBE2O antibody) at dilution of 1:500
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalE2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin- associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853).
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Gene nameUBE2O
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locationCytoplasm,Nucleus
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Uniprot IDQ9C0C9
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Calculated M.W.141kDa,Observed 141kDa，200kDa
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Observed M.W.150 kDa, 200-230 kDa
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