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Alternative name
E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S antibody
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Catalog
A009183
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,FC,IP
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PurificationImmunogen affinity purified
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Immunogenubiquitin-conjugating enzyme E2S
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IHCImmunohistochemistry of paraffin-embedded human breast cancer using A009183(UBE2S antibody) at dilution of 1:50
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Western BlotMDA-MB-453s cells were subjected to SDS PAGE followed by western blot with A009183(UBE2S antibody) at dilution of 1:500
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Recommended dilutionWB : 1:200-1:2000;IP:1:500-1:5000；IHC：1:20-1:200
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Product Description specificalAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.
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Gene nameUBE2S
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locationcytoplasm
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Uniprot IDQ16763
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Calculated M.W.24kDa
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Observed M.W.28 kDa
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