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Alternative name
BRCA1 A complex subunit RAP80, RAP80, Receptor associated protein 80, RXRIP110, UIMC1, X2HRIP110 antibody
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Catalog
A009247
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IF,IHC,IF
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PurificationImmunogen affinity purified
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Immunogenubiquitin interaction motif containing 1
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IHCImmunohistochemistry of paraffin-embedded human testis tissue slide using A009247( UIMC1 Antibody) at dilution of 1:50
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Western Blotmouse colon tissue were subjected to SDS PAGE followed by western blot with A009247(UIMC1 antibody) at dilution of 1:300
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Recommended dilutionWB : 1:200-1:1000;IP:1：200-1：2000；IHC:1：20-1：200;IF:1：10-1：100
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Product Description specificalUbiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.36). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
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Gene nameUIMC1
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locationNucleus
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Uniprot IDQ96RL1
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Calculated M.W.80kDa
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Observed M.W.97 kDa,80 kDa,71-75 kDa,61 kDa
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