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Alternative name
Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13 antibody
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Catalog
A009308
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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Immunogenubiquitin specific peptidase 13 (isopeptidase T-3)
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IHCImmunohistochemistry of paraffin-embedded human testis using A009308(USP13 antibody) at dilution of 1:50
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A009308(USP13 antibody) at dilution of 1:400
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Recommended dilutionWB : 1:500-1:5000;IHC:1:20-1:200;IP:1:200-1:2000
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Product Description specificalDeubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
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Gene nameUSP13
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Uniprot IDQ92995
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Calculated M.W.97kDa
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Observed M.W.97-100 kDa
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