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Alternative name
Deubiquitinating enzyme 16, Ubiquitin thiolesterase 16, UBP M, USP16 antibody
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Catalog
A009312
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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Immunogenubiquitin specific peptidase 16
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IHCImmunohistochemistry of paraffin-embedded human kidney using A009312(USP16 antibody) at dilution of 1:100
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A009312(USP16 antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:500-1:5000;IP:1:500-1:5000;IHC:1:20-1:200
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Product Description specificalSpecifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T- lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
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Gene nameUSP16
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locationNucleus
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Uniprot IDQ9Y5T5
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Calculated M.W.94kDa
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Observed M.W.100 kDa
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