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Alternative name
Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5 antibody
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Catalog
A009339
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IP
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PurificationImmunogen affinity purified
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Immunogenubiquitin specific peptidase 5 (isopeptidase T)
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Western BlotA549 cells were subjected to SDS PAGE followed by western blot with A009339(USP5 antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:500-1:5000;IP:1：500-1：5000
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Product Description specificalCleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
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Gene nameUSP5
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Uniprot IDP45974
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Calculated M.W.93kDa
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Observed M.W.96-100 kDa
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