-
Alternative name
FLJ36605, VIM, Vimentin antibody
-
Catalog
A009408
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,IF,IHC,WB,FC
-
PurificationImmunogen affinity purified
-
Immunogenvimentin
-
IHCImmunohistochemistry of paraffin-embedded human tonsillitis using A009408(VIM antibody) at dilution of 1:50
-
Western BlotNIH/3T3 cells were subjected to SDS PAGE followed by western blot with A009408(VIM antibody) at dilution of 1:2000
-
Recommended dilutionWB :1:1000-1:10000;IHC:1:20-1:500;IF:1:50-1:500
-
Product Description specificalVimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
-
Gene nameVIM
-
locationCytoplasm,Intermediate filament
-
Uniprot IDP08670
-
Calculated M.W.55kDa
-
Observed M.W.57 kDa
-