-
Alternative name
MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B antibody
-
Catalog
A009446
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,WB,IF,IP
-
PurificationImmunogen affinity purified
-
Immunogenvacuolar protein sorting 4 homolog B (S. cerevisiae)
-
Western BlotY79 cells were subjected to SDS PAGE followed by western blot with A009446(VPS4B antibody) at dilution of 1:500
-
Recommended dilutionWB :1:500-1:5000；IP:1:500-1:5000；IF:1:10-1:100
-
Product Description specificalInvolved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
-
Gene nameVPS4B
-
Uniprot IDO75351
-
Calculated M.W.50kDa
-
Observed M.W.50 kDa
-