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Alternative name
PHA2B, PRKWNK4, WNK4 antibody
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Catalog
A009516
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB
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PurificationImmunogen affinity purified
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ImmunogenWNK lysine deficient protein kinase 4
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Western Blotmouse colon tissue were subjected to SDS PAGE followed by western blot with A009516( WNK4 Antibody) at dilution of 1:600
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalSerine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity).
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Gene nameWNK4
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locationCell junction,Tight junction
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Uniprot IDQ96J92
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Calculated M.W.135kDa,Observed 73kDa
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Observed M.W.70 kDa
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