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Alternative name
86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, FLJ39089, G22P2, KARP 1, KARP1, Ku80, Ku86, KUB2, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, TLAA, XRCC5 antibody
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Catalog
A009555
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB,IHC,IF,IP
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PurificationImmunogen affinity purified
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ImmunogenX-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
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IHCImmunohistochemistry of paraffin-embedded human colon cancer using A009555(XRCC5 antibody) at dilution of 1:100
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Western BlotHepG2 cells were subjected to SDS PAGE followed by western blot with A009555(XRCC5 antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000;IHC:1:20-1:200;IF:1:20-1:200;IP:1:500-1:5000
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Product Description specificalThere are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.
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Gene nameXRCC5
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locationChromosome,Nucleus
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Uniprot IDP13010
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Calculated M.W.83kDa
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Observed M.W.80 kDa
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