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Alternative name
65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog antibody
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Catalog
A009559
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Zebrafish
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Applications Tested/SuitableELISA,IHC,FC,IF,IP,WB
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PurificationImmunogen affinity purified
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ImmunogenYes-associated protein 1, 65kDa
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IHCImmunohistochemistry of paraffin-embedded human ovary tumor using A009559(YAP1 antibody) at dilution of 1:100
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A009559( YAP1 Antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000;IHC:1:50-1:200;IF:1:20-1:200;IP:1:200-1:2000
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Product Description specificalTranscriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750). _x000D_Isoform 2: Isoform 2 and isoform 3 can activate the C- terminal fragment (CTF) of ERBB4 (isoform 3).
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Gene nameYAP1
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locationCytoplasm,Nucleus
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Uniprot IDP46937
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Calculated M.W.54kDa,Observed 69kDa
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Observed M.W.65-70 kDa
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