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Alternative name
BP 8, CBF A, CSDA2, CSDB, DBPB, DNA binding protein B, EFI A, Enhancer factor I subunit A, MDR NF1, NSEP 1, NSEP1, Y box binding protein 1, Y box transcription factor, YB 1, YB1, YBX1 antibody
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Catalog
A009561
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC,IP,IF
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PurificationImmunogen affinity purified
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ImmunogenY box binding protein 1
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IHCImmunohistochemistry of paraffin-embedded human placenta using A009561(YBX1 antibody) at dilution of 1:100
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Western BlotMCF7 cells were subjected to SDS PAGE followed by western blot with A009561(YBX1 antibody) at dilution of 1:500
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Recommended dilutionWB:1:500-1:5000;IP:1:200-1:2000;IHC:1:20-1:200;IF:1:20-1:200
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Product Description specificalMediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro. _x000D_The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
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Gene nameYBX1
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locationCytoplasm,Nucleus,Secreted
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Uniprot IDP67809
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Calculated M.W.36kDa,Observed 50kDa
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Observed M.W.50 kDa
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