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Alternative name
Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1 antibody
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Catalog
A009751
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB,IHC,FC,IF,IP
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PurificationImmunogen affinity purified
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Immunogentight junction protein 1 (zona occludens 1)
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IHCImmunohistochemistry of paraffin-embedded human kidney using A009751(ZO1 antibody) at dilution of 1:100
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Western BlotHepG2 cells were subjected to SDS PAGE followed by western blot with A009751(ZO1 antibody) at dilution of 1:500
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Recommended dilutionWB:1:200-1:2000;IP:1:200-1:2000;IHC:1:50-1:200;IF:1:20-1:200
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Product Description specificalTight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
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Gene nameTJP1
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locationCell junction,Cell membrane
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Uniprot IDQ07157
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Calculated M.W.195kDa
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