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Alternative name
HZW10, KNTC1AP, ZW10 antibody
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Catalog
A009760
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB
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PurificationImmunogen affinity purified
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ImmunogenZW10, kinetochore associated, homolog (Drosophila)
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Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A009760( ZW10 Antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000;IP:1:200-1:2000
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Product Description specificalEssential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:11590237, PubMed:15485811, PubMed:15824131). Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (PubMed:15029241).
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Gene nameZW10
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locationCentromere,Chromosome,Cytoplasm,Cytoskeleton,Endoplasmic reticulum,Kinetochore,Membrane
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Uniprot IDO43264
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Calculated M.W.90kDa
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Observed M.W.88-90 kDa
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