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Catalog
C002162
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Alternative name
High mobility group protein B1 CLIA KIT; High mobility group protein 1 CLIA KIT; HMG-1HMGB1 CLIA KIT; HMG1 CLIA KIT; HMG-1 CLIA KIT
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GeneHMGB-1
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Species ReactivityHuman
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Detection Range31.25~2000pg/mL
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Sensitivity18.75pg/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.
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