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Catalog
C000221
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Alternative name
Protransforming growth factor alpha CLIA KIT; Transforming growth factor alpha CLIA KIT; TGF-alphaTGFA CLIA KIT; TGF-alpha CLIA KIT; ETGF CLIA KIT
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GeneTGFa
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of TGFa. No significant cross-reactivity or interference between TGFa and analogues was observed.
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Detection Range2.74-2,000pg/mL. The standard curve concentrations used for the CLIA's were 2,000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, 8.23pg/mL, 2.74pg/mL.
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SensitivityThe minimum detectable dose of TGFa is typically less than 0.94pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TGFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the TGFa level in the sample or standard.
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