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Catalog
C002369
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Alternative name
Glypican-3 CLIA KIT; GTR2-2 CLIA KIT; Intestinal protein OCI-5 CLIA KIT; MXR7GPC3 CLIA KIT; OCI5 CLIA KIT
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GeneGPC3
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant GPC3. No significant cross-reactivity or interference between GPC3 and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range31.25-2000pg/mL
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Sensitivity18.75pg/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of GPC3 concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to GPC3. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of GPC3. You can calculate the concentration of GPC3 in the samples by comparing the RLU value of the samples to the standard curve.
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