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Catalog
C000025
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Alternative name
Zinc finger E-box-binding homeobox 2 CLIA KIT; Smad-interacting protein 1 CLIA KIT; SMADIP1 CLIA KIT; Zinc finger homeobox protein 1bZEB2 CLIA KIT; KIAA0569 CLIA KIT; SIP1 CLIA KIT; ZFHX1B CLIA KIT; ZFX1B CLIA KIT; SMADIP1 CLIA KIT
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GeneZFHX1B
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Zinc Finger Homeobox Protein 1B (ZFHX1B).
No significant cross-reactivity or interference between Zinc Finger Homeobox Protein 1B (ZFHX1B) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range13.72-10000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 6.45pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Zinc Finger Homeobox Protein 1B (ZFHX1B) level in the sample or standard.
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