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Catalog
C000326
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Alternative name
Tissue factor pathway inhibitor CLIA KIT; Extrinsic pathway inhibitor CLIA KIT; EPI CLIA KIT; Lipoprotein-associated coagulation inhibitor CLIA KIT; LACITFPI CLIA KIT; TFPI CLIA KIT; EPI CLIA KIT; LACI CLIA KIT
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GeneTFPI
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of TFPI. No significant cross-reactivity or interference between TFPI and analogues was observed.
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Detection Range27.4-20,000pg/mL. The standard curve concentrations used for the CLIA's were 20,000pg/mL, 6,666.7pg/mL, 2,222.2pg/mL, 740.7pg/mL, 246.9pg/mL, 82.3pg/mL, 27.4pg/mL.
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SensitivityThe minimum detectable dose of TFPI is typically less than 12.4pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TFPI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TFPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the TFPI level in the sample or standard.
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