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Catalog
C000648
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Alternative name
Protein S100-A12 CLIA KIT; CGRP CLIA KIT; Calcium-binding protein in amniotic fluid 1 CLIA KIT; CAAF1S100A12 CLIA KIT; CAAF1 CLIA KIT; CAGC CLIA KIT; EN-RAGE CLIA KIT; MRP-6 CLIA KIT; p6 CLIA KIT
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GeneS100A12
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant S100A12. No significant cross-reactivity or interference between S100A12 and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Sensitivity18.75pg/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of S100A12 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to S100A12. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100A12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100A12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of S100A12. You can calculate the concentration of S100A12 in the samples by comparing the RLU value of the samples to the standard curve.
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