Human CYP2C9 ELISA Kit

Alternative name
Human (R)-limonene 6-monooxygenase ELISA Kit;Human (S)-limonene 6-monooxygenase ELISA Kit;Human (S)-limonene 7-monooxygenase ELISA Kit;Human CYPIIC9 ELISA Kit;Human Cholesterol 25-hydroxylase ELISA Kit;Human cytochrome P-450MP ELISA Kit;Human Cytochrome P450 MP-4 ELISA Kit;Human Cytochrome P450 MP-8 ELISA Kit;Human cytochrome P450 PB-1 ELISA Kit;Human S-mephenytoin 4-hydroxylase ELISA Kit;Human CYP2C10 ELISA Kit;Human CPC9 ELISA Kit;Human CYP2C ELISA Kit;Human P450IIC9 ELISA Kit;Human cytochrome P450 family 2 subfamily C member 9 ELISA Kit;Human cytochrome P450 2C9 ELISA Kit;Human cytochrome P-450 S-mephenytoin 4-hydroxylase ELISA Kit;Human cytochrome P450, family 2, subfamily C, polypeptide 9 ELISA Kit;Human flavoprotein-linked monooxygenase ELISA Kit;Human microsomal monooxygenase ELISA Kit;Human xenobiotic monooxygenase ELISA Kit;
Catalog
E011057
species
Human
GeneCYP2C9
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 2C9 (CYP2C9). No significant cross-reactivity or interference between Cytochrome P450 2C9 (CYP2C9) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.253ng/mL.
Intended UseHuman CYP2C9 ELISA Kit allows for the in vitro quantitative determination of CYP2C9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2C9 (CYP2C9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 2C9 (CYP2C9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2C9 (CYP2C9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2C9 (CYP2C9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytochrome P450 2C9 Protein information
Uniprot ID CP2C9_HUMAN
Uniprot AC P11712; P11713; Q16756; Q16872; Q5VX92; Q6IRV8; Q8WW80;
UniGene Hs.282624;
GeneID 1559
KEGG hsa:1559;
Human Cytochrome P450 2C9 Protein SEQUENCE
SEQUENCE 490 AA; 55628 MW; 4FDFC395303A4E3E CRC64;
MDSLVVLVLC LSCLLLLSLW RQSSGRGKLP PGPTPLPVIG NILQIGIKDI
SKSLTNLSKV YGPVFTLYFG LKPIVVLHGY EAVKEALIDL GEEFSGRGIF
PLAERANRGF GIVFSNGKKW KEIRRFSLMT LRNFGMGKRS IEDRVQEEAR
CLVEELRKTK ASPCDPTFIL GCAPCNVICS IIFHKRFDYK DQQFLNLMEK
LNENIKILSS PWIQICNNFS PIIDYFPGTH NKLLKNVAFM KSYILEKVKE
HQESMDMNNP QDFIDCFLMK MEKEKHNQPS EFTIESLENT AVDLFGAGTE
TTSTTLRYAL LLLLKHPEVT AKVQEEIERV IGRNRSPCMQ DRSHMPYTDA
VVHEVQRYID LLPTSLPHAV TCDIKFRNYL IPKGTTILIS LTSVLHDNKE
FPNPEMFDPH HFLDEGGNFK KSKYFMPFSA GKRICVGEAL AGMELFLFLT
SILQNFNLKS LVDPKNLDTT PVVNGFASVP PFYQLCFIPV

Category

Human CYP2C9 ELISA Kit