Human D2HGDH ELISA Kit

Alternative name
Human D2HGD ELISA Kit;Human D-2-hydroxyglutarate dehydrogenase ELISA Kit;Human D-2-hydroxyglutarate dehydrogenase, mitochondrial ELISA Kit;
Catalog
E011351
species
Human
GeneD2HGDH
SpecificityThis assay has high sensitivity and excellent specificity for detection of D2-Hydroxyglutarate Dehydrogenase (D2HGDH). No significant cross-reactivity or interference between D2-Hydroxyglutarate Dehydrogenase (D2HGDH) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman D2HGDH ELISA Kit allows for the in vitro quantitative determination of D2HGDH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D2-Hydroxyglutarate Dehydrogenase (D2HGDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D2-Hydroxyglutarate Dehydrogenase (D2HGDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D2-Hydroxyglutarate Dehydrogenase (D2HGDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D2-Hydroxyglutarate Dehydrogenase (D2HGDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human D-2-hydroxyglutarate dehydrogenase, mitochondrial Protein information
Uniprot ID D2HDH_HUMAN
Uniprot AC Q8N465; B4E3L6; E7ENP2; Q6IQ24; Q8N5Q8;
UniGene Hs.516813;
GeneID 728294
KEGG hsa:728294;
Human D-2-hydroxyglutarate dehydrogenase, mitochondrial Protein SEQUENCE
SEQUENCE 521 AA; 56416 MW; 65D88C4315FA45CE CRC64;
MLPRRPLAWP AWLLRGAPGA AGSWGRPVGP LARRGCCSAP GTPEVPLTRE
RYPVRRLPFS TVSKQDLAAF ERIVPGGVVT DPEALQAPNV DWLRTLRGCS
KVLLRPRTSE EVSHILRHCH ERNLAVNPQG GNTGMVGGSV PVFDEIILST
ARMNRVLSFH SVSGILVCQA GCVLEELSRY VEERDFIMPL DLGAKGSCHI
GGNVATNAGG LRFLRYGSLH GTVLGLEVVL ADGTVLDCLT SLRKDNTGYD
LKQLFIGSEG TLGIITTVSI LCPPKPRAVN VAFLGCPGFA EVLQTFSTCK
GMLGEILSAF EFMDAVCMQL VGRHLHLASP VQESPFYVLI ETSGSNAGHD
AEKLGHFLEH ALGSGLVTDG TMATDQRKVK MLWALRERIT EALSRDGYVY
KYDLSLPVER LYDIVTDLRA RLGPHAKHVV GYGHLGDGNL HLNVTAEAFS
PSLLAALEPH VYEWTAGQQG SVSAEHGVGF RKRDVLGYSK PPGALQLMQQ
LKALLDPKGI LNPYKTLPSQ A
UCSC uc002wce.3; human. [Q8N465-1];

Category

Human D2HGDH ELISA Kit

Human D2HGD ELISA Kit;Human D-2-hydroxyglutarate dehydrogenase ELISA Kit;Human D-2-hydroxyglutarate dehydrogenase, mitochondrial ELISA Kit;