Human DGAT1 ELISA Kit

Alternative name
Human ACAT-related gene product 1 ELISA Kit;Human acyl-CoA retinol O-fatty-acyltransferase ELISA Kit;Human ARAT ELISA Kit;Human Retinol O-fatty-acyltransferase ELISA Kit;Human diglyceride acyltransferase ELISA Kit;Human AGRP1 ELISA Kit;Human DGAT ELISA Kit;Human ARGP1 ELISA Kit;Human DIAR7 ELISA Kit;Human diacylglycerol O-acyltransferase 1 ELISA Kit;Human ACAT related gene product 1 ELISA Kit;Human acyl coenzyme A:cholesterol acyltransferase related gene 1 ELISA Kit;Human acyl-CoA:diacylglycerol acyltransferase ELISA Kit;
Catalog
E011829
species
Human
GeneDGAT1
SpecificityThis assay has high sensitivity and excellent specificity for detection of DGAT1 . No significant cross-reactivity or interference between DGAT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 11.2pg/ml
Intended UseHuman DGAT1 ELISA Kit allows for the in vitro quantitative determination of DGAT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DGAT1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DGAT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DGAT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DGAT1 can be calculated.
Human Diacylglycerol O-acyltransferase 1 Protein information
Uniprot ID DGAT1_HUMAN
Uniprot AC O75907; B2RWQ2; D3DWL6; Q96BB8;
UniGene Hs.521954;
GeneID 8694
KEGG hsa:8694;
Human Diacylglycerol O-acyltransferase 1 Protein SEQUENCE
SEQUENCE 488 AA; 55278 MW; 6574D5DBF15D6171 CRC64;
MGDRGSSRRR RTGSRPSSHG GGGPAAAEEE VRDAAAGPDV GAAGDAPAPA
PNKDGDAGVG SGHWELRCHR LQDSLFSSDS GFSNYRGILN WCVVMLILSN
ARLFLENLIK YGILVDPIQV VSLFLKDPYS WPAPCLVIAA NVFAVAAFQV
EKRLAVGALT EQAGLLLHVA NLATILCFPA AVVLLVESIT PVGSLLALMA
HTILFLKLFS YRDVNSWCRR ARAKAASAGK KASSAAAPHT VSYPDNLTYR
DLYYFLFAPT LCYELNFPRS PRIRKRFLLR RILEMLFFTQ LQVGLIQQWM
VPTIQNSMKP FKDMDYSRII ERLLKLAVPN HLIWLIFFYW LFHSCLNAVA
ELMQFGDREF YRDWWNSESV TYFWQNWNIP VHKWCIRHFY KPMLRRGSSK
WMARTGVFLA SAFFHEYLVS VPLRMFRLWA FTGMMAQIPL AWFVGRFFQG
NYGNAAVWLS LIIGQPIAVL MYVHDYYVLN YEAPAAEA
UCSC uc003zbv.5; human.;

Category

Human DGAT1 ELISA Kit