Human DYRK1A ELISA Kit

Alternative name
Human dual specificity YAK1-related kinase ELISA Kit;Human HP86 ELISA Kit;Human protein kinase minibrain homolog ELISA Kit;Human MNBH ELISA Kit;Human hMNB ELISA Kit;Human DYRK ELISA Kit;Human MNB ELISA Kit;Human DYRK1 ELISA Kit;Human MRD7 ELISA Kit;Human dual specificity tyrosine phosphorylation regulated kinase 1A ELISA Kit;Human dual specificity tyrosine-phosphorylation-regulated kinase 1A ELISA Kit;Human MNB/DYRK protein kinase ELISA Kit;Human dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A ELISA Kit;Human mnb protein kinase homolog hp86 ELISA Kit;Human serine/threonine kinase MNB ELISA Kit;Human serine/threonine-specific protein kinase ELISA Kit;
Catalog
E012474
species
Human
GeneDYRK1A
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). No significant cross-reactivity or interference between Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.117ng/mL.
Intended UseHuman DYRK1A ELISA Kit allows for the in vitro quantitative determination of DYRK1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A Protein information
Uniprot ID DYR1A_HUMAN
Uniprot AC Q13627; O60769; Q92582; Q92810; Q9UNM5;
UniGene Hs.368240;
GeneID 1859
KEGG hsa:1859;
Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A Protein SEQUENCE
SEQUENCE 763 AA; 85584 MW; 7C3A52A3CBB04FB5 CRC64;
MHTGGETSAC KPSSVRLAPS FSFHAAGLQM AGQMPHSHQY SDRRQPNISD
QQVSALSYSD QIQQPLTNQV MPDIVMLQRR MPQTFRDPAT APLRKLSVDL
IKTYKHINEV YYAKKKRRHQ QGQGDDSSHK KERKVYNDGY DDDNYDYIVK
NGEKWMDRYE IDSLIGKGSF GQVVKAYDRV EQEWVAIKII KNKKAFLNQA
QIEVRLLELM NKHDTEMKYY IVHLKRHFMF RNHLCLVFEM LSYNLYDLLR
NTNFRGVSLN LTRKFAQQMC TALLFLATPE LSIIHCDLKP ENILLCNPKR
SAIKIVDFGS SCQLGQRIYQ YIQSRFYRSP EVLLGMPYDL AIDMWSLGCI
LVEMHTGEPL FSGANEVDQM NKIVEVLGIP PAHILDQAPK ARKFFEKLPD
GTWNLKKTKD GKREYKPPGT RKLHNILGVE TGGPGGRRAG ESGHTVADYL
KFKDLILRML DYDPKTRIQP YYALQHSFFK KTADEGTNTS NSVSTSPAME
QSQSSGTTSS TSSSSGGSSG TSNSGRARSD PTHQHRHSGG HFTAAVQAMD
CETHSPQVRQ QFPAPLGWSG TEAPTQVTVE THPVQETTFH VAPQQNALHH
HHGNSSHHHH HHHHHHHHHG QQALGNRTRP RVYNSPTNSS STQDSMEVGH
SHHSMTSLSS STTSSSTSSS STGNQGNQAY QNRPVAANTL DFGQNGAMDV
NLTVYSNPRQ ETGIAGHPTY QFSANTGPAH YMTEGHLTMR QGADREESPM
TGVCVQQSPV ASS
UCSC uc002ywi.4; human. [Q13627-1];

Category

Human DYRK1A ELISA Kit