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Alternative name
Human elastase-specific inhibitor ELISA Kit;Human ESI ELISA Kit;Human peptidase inhibitor 3 ELISA Kit;Human PI-3 ELISA Kit;Human protease inhibitor WAP3 ELISA Kit;Human skin-derived antileukoproteinase ELISA Kit;Human SKALP ELISA Kit;Human WAP four-disulfide core domain protein 14 ELISA Kit;Human WAP3 ELISA Kit;Human WFDC14 ELISA Kit;Human cementoin ELISA Kit;Human elafin ELISA Kit;Human WAP four-disulfide core domain 14 ELISA Kit;Human peptidase inhibitor 3, skin-derived ELISA Kit;Human pre-elafin ELISA Kit;Human protease inhibitor 3, skin-derived (SKALP) ELISA Kit;Human trappin-2 ELISA Kit;
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Catalog
E012852
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species
Human
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GenePI3
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SpecificityNatural and recombinant human Elafin
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 10pg/ml
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Intended UseHuman PI3 ELISA Kit allows for the in vitro quantitative determination of PI3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human Elafin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Elafin has been precoated onto 96-well plates. Standards(NSO, A23-Q117) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Elafin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Elafin amount of sample captured in plate.
Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
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Human Elafin Protein information
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Uniprot ID
ELAF_HUMAN
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Uniprot AC
P19957; E1P618; Q6FG74;
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UniGene
Hs.112341;
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GeneID
5266
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KEGG
hsa:5266;
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Human Elafin Protein SEQUENCE
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SEQUENCE 117 AA; 12270 MW; A51D46257E5B03EF CRC64;
MRASSFLIVV VFLIAGTLVL EAAVTGVPVK GQDTVKGRVP FNGQDPVKGQ
VSVKGQDKVK
AQEPVKGPVS TKPGSCPIIL IRCAMLNPPN RCLKDTDCPG
IKKCCEGSCG MACFVPQ
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UCSC
uc002xng.4; human.;
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