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Alternative name
Chymotrypsin-like elastase family member 1 ELISA KIT; Elastase-1 ELISA KIT; Pancreatic elastase 1CELA1 ELISA KIT; ELA1 ELISA KIT
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Catalog
E012860
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species
Human
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GeneELA1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ELA1. No significant cross-reactivity or interference between Human ELA1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.156 ng/ml
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Intended UseHuman ELA1 ELISA Kit allows for the in vitro quantitative determination of ELA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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