Human EVPL ELISA Kit

Alternative name
Human 210 kDa cornified envelope precursor protein ELISA Kit;Human 210 kDa paraneoplastic pemphigus antigen ELISA Kit;Human p210 ELISA Kit;Human EVPK ELISA Kit;Human envoplakin ELISA Kit;
Catalog
E013227
species
Human
GeneEVPL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Envoplakin (EVPL). No significant cross-reactivity or interference between Envoplakin (EVPL) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman EVPL ELISA Kit allows for the in vitro quantitative determination of EVPL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Envoplakin (EVPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Envoplakin (EVPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Envoplakin (EVPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Envoplakin (EVPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Envoplakin Protein information
Uniprot ID EVPL_HUMAN
Uniprot AC Q92817; A0AUV5;
UniGene Hs.500635;
GeneID 2125
KEGG hsa:2125;
Human Envoplakin Protein SEQUENCE
SEQUENCE 2033 AA; 231604 MW; 0A994627703393E0 CRC64;
MFKGLSKGSQ GKGSPKGSPA KGSPKGSPSR HSRAATQELA LLISRMQANA
DQVERDILET QKRLQQDRLN SEQSQALQHQ QETGRSLKEA EVLLKDLFLD
VDKARRLKHP QAEEIEKDIK QLHERVTQEC AEYRALYEKM VLPPDVGPRV
DWARVLEQKQ KQVCAGQYGP GMAELEQQIA EHNILQKEID AYGQQLRSLV
GPDAATIRSQ YRDLLKAASW RGQSLGSLYT HLQGCTRQLS ALAEQQRRIL
QQDWSDLMAD PAGVRREYEH FKQHELLSQE QSVNQLEDDG ERMVELRHPA
VGPIQAHQEA LKMEWQNFLN LCICQETQLQ HVEDYRRFQE EADSVSQTLA
KLNSNLDAKY SPAPGGPPGA PTELLQQLEA EEKRLAVTER ATGDLQRRSR
DVAPLPQRRN PPQQPLHVDS ICDWDSGEVQ LLQGERYKLV DNTDPHAWVV
QGPGGETKRA PAACFCIPAP DPDAVARASR LASELQALKQ KLATVQSRLK
ASAVESLRPS QQAPSGSDLA NPQAQKLLTQ MTRLDGDLGQ IERQVLAWAR
APLSRPTPLE DLEGRIHSHE GTAQRLQSLG TEKETAQKEC EAFLSTRPVG
PAALQLPVAL NSVKNKFSDV QVLCSLYGEK AKAALDLERQ IQDADRVIRG
FEATLVQEAP IPAEPGALQE RVSELQRQRR ELLEQQTCVL RLHRALKASE
HACAALQNNF QEFCQDLPRQ QRQVRALTDR YHAVGDQLDL REKVVQDAAL
TYQQFKNCKD NLSSWLEHLP RSQVRPSDGP SQIAYKLQAQ KRLTQEIQSR
ERDRATASHL SQALQAALQD YELQADTYRC SLEPTLAVSA PKRPRVAPLQ
ESIQAQEKNL AKAYTEVAAA QQQLLQQLEF ARKMLEKKEL SEDIRRTHDA
KQGSESPAQA GRESEALKAQ LEEERKRVAR VQHELEAQRS QLLQLRTQRP
LERLEEKEVV EFYRDPQLEG SLSRVKAQVE EEGKRRAGLQ ADLEVAAQKV
VQLESKRKTM QPHLLTKEVT QVERDPGLDS QAAQLRIQIQ QLRGEDAVIS
ARLEGLKKEL LALEKREVDV KEKVVVKEVV KVEKNLEMVK AAQALRLQME
EDAARRKQAE EAVAKLQARI EDLERAISSV EPKVIVKEVK KVEQDPGLLQ
ESSRLRSLLE EERTKNATLA RELSDLHSKY SVVEKQRPKV QLQERVHEIF
QVDPETEQEI TRLKAKLQEM AGKRSGVEKE VEKLLPDLEV LRAQKPTVEY
KEVTQEVVRH ERSPEVLREI DRLKAQLNEL VNSHGRSQEQ LIRLQGERDE
WRRERAKVET KTVSKEVVRH EKDPVLEKEA ERLRQEVREA AQKRRAAEDA
VYELQSKRLL LERRKPEEKV VVQEVVVTQK DPKLREEHSR LSGSLDEEVG
RRRQLELEVQ QLRAGVEEQE GLLSFQEDRS KKLAVERELR QLTLRIQELE
KRPPTVQEKI IMEEVVKLEK DPDLEKSTEA LRWDLDQEKT QVTELNRECK
NLQVQIDVLQ KAKSQEKTIY KEVIRVQKDR VLEDERARVW EMLNRERTAR
QAREEEARRL RERIDRAETL GRTWSREESE LQRARDQADQ ECGRLQQELR
ALERQKQQQT LQLQEESKLL SQKTESERQK AAQRGQELSR LEAAILREKD
QIYEKERTLR DLHAKVSREE LSQETQTRET NLSTKISILE PETGKDMSPY
EAYKRGIIDR GQYLQLQELE CDWEEVTTSG PCGEESVLLD RKSGKQYSIE
AALRCRRISK EEYHLYKDGH LPISEFALLV AGETKPSSSL SIGSIISKSP
LASPAPQSTS FFSPSFSLGL GDDSFPIAGI YDTTTDNKCS IKTAVAKNML
DPITGQKLLE AQAATGGIVD LLSRERYSVH KAMERGLIEN TSTQRLLNAQ
KAFTGIEDPV TKKRLSVGEA VQKGWMPRES VLPHLQVQHL TGGLIDPKRT
GRIPIQQALL SGMISEELAQ LLQDESSYEK DLTDPISKER LSYKEAMGRC
RKDPLSGLLL LPAALEGYRC YRSASPTVPR SLR
UCSC uc002jqi.3; human.;

Category

Human EVPL ELISA Kit

Human 210 kDa cornified envelope precursor protein ELISA Kit;Human 210 kDa paraneoplastic pemphigus antigen ELISA Kit;Human p210 ELISA Kit;Human EVPK ELISA Kit;Human envoplakin ELISA Kit;