Human NPHS1 ELISA Kit

Alternative name
Human renal glomerulus-specific cell adhesion receptor ELISA Kit;Human NPHN ELISA Kit;Human CNF ELISA Kit;Human nephrin ELISA Kit;Human NPHS1, nephrin ELISA Kit;Human nephrosis 1, congenital, Finnish type (nephrin) ELISA Kit;Human truncated NPHS1 ELISA Kit;
Catalog
E025961
species
Human
GeneNPHS1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NPHS1. No significant cross-reactivity or interference between Human NPHS1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.039 ng/ml
Intended UseHuman NPHS1 ELISA Kit allows for the in vitro quantitative determination of NPHS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Nephrin is a protein necessary for the proper functioning of therenal filtration barrier. The renal filtration barrier consists offenestrated endothelial cells, the glomerular basementmembrane, and the podocytes of epithelial cells. Nephrin is atransmembrane protein involved with the slit diaphragm. A defectin the gene for nephrin is associated with congenital nephroticsyndrome of the Finnish type and causes massive amounts ofprotein to be leaked into the urine, or proteinuria. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated withan antibody specific to nephrin. Standards or samples are thenadded to the appropriate microtiter plate wells with abiotin-conjugated antibody preparation specific for nephrin andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain nephrin, biotin-conjugated antibodyand enzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of nephrin in the samples is then determined bycomparing the O.D. of the samples to the standard curve.
Human Nephrin Protein information
Uniprot ID NPHN_HUMAN
Uniprot AC O60500; A6NDH2; C3RX61;
UniGene Hs.122186;
GeneID 4868
KEGG hsa:4868;
Human Nephrin Protein SEQUENCE
SEQUENCE 1241 AA; 134742 MW; 7F5AFAF078BCF532 CRC64;
MALGTTLRAS LLLLGLLTEG LAQLAIPASV PRGFWALPEN LTVVEGASVE
LRCGVSTPGS AVQWAKDGLL LGPDPRIPGF PRYRLEGDPA RGEFHLHIEA
CDLSDDAEYE CQVGRSEMGP ELVSPRVILS ILVPPKLLLL TPEAGTMVTW
VAGQEYVVNC VSGDAKPAPD ITILLSGQTI SDISANVNEG SQQKLFTVEA
TARVTPRSSD NRQLLVCEAS SPALEAPIKA SFTVNVLFPP GPPVIEWPGL
DEGHVRAGQS LELPCVARGG NPLATLQWLK NGQPVSTAWG TEHTQAVARS
VLVMTVRPED HGAQLSCEAH NSVSAGTQEH GITLQVTFPP SAIIILGSAS
QTENKNVTLS CVSKSSRPRV LLRWWLGWRQ LLPMEETVMD GLHGGHISMS
NLTFLARRED NGLTLTCEAF SEAFTKETFK KSLILNVKYP AQKLWIEGPP
EGQKLRAGTR VRLVCLAIGG NPEPSLMWYK DSRTVTESRL PQESRRVHLG
SVEKSGSTFS RELVLVTGPS DNQAKFTCKA GQLSASTQLA VQFPPTNVTI
LANASALRPG DALNLTCVSV SSNPPVNLSW DKEGERLEGV AAPPRRAPFK
GSAAARSVLL QVSSRDHGQR VTCRAHSAEL RETVSSFYRL NVLYRPEFLG
EQVLVVTAVE QGEALLPVSV SANPAPEAFN WTFRGYRLSP AGGPRHRILS
SGALHLWNVT RADDGLYQLH CQNSEGTAEA RLRLDVHYAP TIRALQDPTE
VNVGGSVDIV CTVDANPILP GMFNWERLGE DEEDQSLDDM EKISRGPTGR
LRIHHAKLAQ AGAYQCIVDN GVAPPARRLL RLVVRFAPQV EHPTPLTKVA
AAGDSTSSAT LHCRARGVPN IVFTWTKNGV PLDLQDPRYT EHTYHQGGVH
SSLLTIANVS AAQDYALFTC TATNALGSDQ TNIQLVSISR PDPPSGLKVV
SLTPHSVGLE WKPGFDGGLP QRFCIRYEAL GTPGFHYVDV VPPQATTFTL
TGLQPSTRYR VWLLASNALG DSGLADKGTQ LPITTPGLHQ PSGEPEDQLP
TEPPSGPSGL PLLPVLFALG GLLLLSNASC VGGVLWQRRL RRLAEGISEK
TEAGSEEDRV RNEYEESQWT GERDTQSSTV STTEAEPYYR SLRDFSPQLP
PTQEEVSYSR GFTGEDEDMA FPGHLYDEVE RTYPPSGAWG PLYDEVQMGP
WDLHWPEDTY QDPRGIYDQV AGDLDTLEPD SLPFELRGHL V
UCSC uc002oby.4; human. [O60500-1];

Category

Human NPHS1 ELISA Kit

Human renal glomerulus-specific cell adhesion receptor ELISA Kit;Human NPHN ELISA Kit;Human CNF ELISA Kit;Human nephrin ELISA Kit;Human NPHS1, nephrin ELISA Kit;Human nephrosis 1, congenital, Finnish type (nephrin) ELISA Kit;Human truncated NPHS1 ELISA Kit;