Human EEF1G ELISA Kit

Alternative name
Human EF-1-gamma ELISA Kit;Human eEF-1B gamma ELISA Kit;Human EF1G ELISA Kit;Human GIG35 ELISA Kit;Human eukaryotic translation elongation factor 1 gamma ELISA Kit;Human elongation factor 1-gamma ELISA Kit;Human PRO1608 ELISA Kit;Human pancreatic tumor-related protein ELISA Kit;Human translation elongation factor eEF-1 gamma chain ELISA Kit;
Catalog
E013719
species
Human
GeneEEF1G
SpecificityThis assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). No significant cross-reactivity or interference between Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 12.4pg/mL.
Intended UseHuman EEF1G ELISA Kit allows for the in vitro quantitative determination of EEF1G , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Elongation factor 1-gamma Protein information
Uniprot ID EF1G_HUMAN
Uniprot AC P26641; B4DTG2; Q6PJ62; Q6PK31; Q96CU2; Q9P196;
UniGene Hs.144835; Hs.444467;
GeneID 1937
KEGG hsa:1937;
Human Elongation factor 1-gamma Protein SEQUENCE
SEQUENCE 437 AA; 50119 MW; A6110663110CF3FC CRC64;
MAAGTLYTYP ENWRAFKALI AAQYSGAQVR VLSAPPHFHF GQTNRTPEFL
RKFPAGKVPA FEGDDGFCVF ESNAIAYYVS NEELRGSTPE AAAQVVQWVS
FADSDIVPPA STWVFPTLGI MHHNKQATEN AKEEVRRILG LLDAYLKTRT
FLVGERVTLA DITVVCTLLW LYKQVLEPSF RQAFPNTNRW FLTCINQPQF
RAVLGEVKLC EKMAQFDAKK FAETQPKKDT PRKEKGSREE KQKPQAERKE
EKKAAAPAPE EEMDECEQAL AAEPKAKDPF AHLPKSTFVL DEFKRKYSNE
DTLSVALPYF WEHFDKDGWS LWYSEYRFPE ELTQTFMSCN LITGMFQRLD
KLRKNAFASV ILFGTNNSSS ISGVWVFRGQ ELAFPLSPDW QVDYESYTWR
KLDPGSEETQ TLVREYFSWE GAFQHVGKAF NQGKIFK

Category

Human EEF1G ELISA Kit