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Human ERRa ELISA Kit

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  • Alternative name

    Steroid hormone receptor ERR1 ELISA KIT; Estrogen receptor-like 1 ELISA KIT; Estrogen-related receptor alpha ELISA KIT; ERR-alpha ELISA KIT; Nuclear receptor subfamily 3 group B member 1ESRRA ELISA KIT; ERR1 ELISA KIT; ESRL1 ELISA KIT; NR3B1 ELISA KIT; ERR-alpha ELISA KIT
  • Catalog
    E013657
  • species
    Human
  • GeneERRa
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Estrogen Related Receptor Alpha (ERRa). No significant cross-reactivity or interference between Estrogen Related Receptor Alpha (ERRa) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.127ng/mL.
  • Intended UseHuman ERRa ELISA Kit allows for the in vitro quantitative determination of ERRa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Related Receptor Alpha (ERRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Related Receptor Alpha (ERRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Related Receptor Alpha (ERRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Related Receptor Alpha (ERRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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