Mouse Hdgf ELISA Kit

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  • Alternative name

    Mouse HDGF ELISA Kit;Mouse Tdrm1 ELISA Kit;Mouse AI118077 ELISA Kit;Mouse D3Ertd299e ELISA Kit;Mouse hepatoma-derived growth factor ELISA Kit;

  • Catalog
    E055213
  • species
    Mouse
  • GeneHdgf
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse HDGF. No significant cross-reactivity or interference between Mouse HDGF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse HDGF and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity9.375pg/mL
  • Intended UseMouse Hdgf ELISA Kit allows for the in vitro quantitative determination of Hdgf , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HDGF in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HDGF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse HDGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HDGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse HDGF. You can calculate the concentration of Mouse HDGF in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Hepatoma-derived growth factor Protein information
  • Uniprot ID HDGF_MOUSE
  • Uniprot AC P51859; Q8BPG7; Q9CYA4; Q9JK87;
  • UniGene Mm.292208;
  • GeneID 15191
  • KEGG mmu:15191;
  • Mouse Hepatoma-derived growth factor Protein SEQUENCE
  • SEQUENCE 237 AA; 26269 MW; AAE4CF574DA4733F CRC64;

    MSRSNRQKEY KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF

    GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY

    QSSQKKSCAA EPEVEPEAHE GDGDKKGSAE GSSDEEGKLV IDEPAKEKNE

    KGTLKRRAGD VLEDSPKRPK ESGDHEEEDK EIAALEGERP LPVEVEKNST

    PSEPDSGQGP PAEEEEGEEE AAKEEAEAQG VRDHESL

  • UCSC uc008ptc.2; mouse.;



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