Human FOXO4 ELISA Kit

Alternative name
Human fork head domain transcription factor AFX1 ELISA Kit;Human AFX ELISA Kit;Human AFX1 ELISA Kit;Human MLLT7 ELISA Kit;Human forkhead box O4 ELISA Kit;Human forkhead box protein O4 ELISA Kit;Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 ELISA Kit;
Catalog
E015476
species
Human
GeneFOXO4
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human FOXO4. No significant cross-reactivity or interference between Human FOXO4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human FOXO4 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman FOXO4 ELISA Kit allows for the in vitro quantitative determination of FOXO4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FOXO4 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FOXO4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human FOXO4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FOXO4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FOXO4. You can calculate the concentration of Human FOXO4 in the samples by comparing the OD of the samples to the standard curve.
Human Forkhead box protein O4 Protein information
Uniprot ID FOXO4_HUMAN
Uniprot AC P98177; B7WPJ7; O43821; Q13720; Q3KPF1; Q8TDK9;
UniGene Hs.584654;
GeneID 4303
KEGG hsa:4303;
Human Forkhead box protein O4 Protein SEQUENCE
SEQUENCE 505 AA; 53684 MW; 0C71C8E2167CEE68 CRC64;
MDPGNENSAT EAAAIIDLDP DFEPQSRPRS CTWPLPRPEI ANQPSEPPEV
EPDLGEKVHT EGRSEPILLP SRLPEPAGGP QPGILGAVTG PRKGGSRRNA
WGNQSYAELI SQAIESAPEK RLTLAQIYEW MVRTVPYFKD KGDSNSSAGW
KNSIRHNLSL HSKFIKVHNE ATGKSSWWML NPEGGKSGKA PRRRAASMDS
SSKLLRGRSK APKKKPSVLP APPEGATPTS PVGHFAKWSG SPCSRNREEA
DMWTTFRPRS SSNASSVSTR LSPLRPESEV LAEEIPASVS SYAGGVPPTL
NEGLELLDGL NLTSSHSLLS RSGLSGFSLQ HPGVTGPLHT YSSSLFSPAE
GPLSAGEGCF SSSQALEALL TSDTPPPPAD VLMTQVDPIL SQAPTLLLLG
GLPSSSKLAT GVGLCPKPLE APGPSSLVPT LSMIAPPPVM ASAPIPKALG
TPVLTPPTEA ASQDRMPQDL DLDMYMENLE CDMDNIISDL MDEGEGLDFN
FEPDP
UCSC uc004dys.3; human. [P98177-1];

Category

Human FOXO4 ELISA Kit