Mouse Bmp4 ELISA Kit

Alternative name
Mouse Bmp-4 ELISA Kit;Mouse bone morphogenetic protein 2B ELISA Kit;Mouse BMP-2B ELISA Kit;Mouse Dvr-4 ELISA Kit;Mouse Bmp2b ELISA Kit;Mouse Bmp2b-1 ELISA Kit;Mouse Bmp2b1 ELISA Kit;Mouse bone morphogenetic protein 4 ELISA Kit;
Catalog
E045925
species
Mouse
GeneBmp4
SpecificityNatural and recombinant mouse BMP-4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of mouse BMP-4 in bone tissue, cell culture supernates an
Intended UseMouse Bmp4 ELISA Kit allows for the in vitro quantitative determination of Bmp4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Mouse BMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-4 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-4 amount of sample captured in plate. Background: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Also, BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate.
Mouse Bone morphogenetic protein 4 Protein information
Uniprot ID BMP4_MOUSE
Uniprot AC P21275;
UniGene Mm.6813;
GeneID 12159
KEGG mmu:12159;
Mouse Bone morphogenetic protein 4 Protein SEQUENCE
SEQUENCE 408 AA; 46497 MW; 35053D844624EF9D CRC64;
MIPGNRMLMV VLLCQVLLGG ASHASLIPET GKKKVAEIQG HAGGRRSGQS
HELLRDFEAT LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEEQS
QGTGLEYPER PASRANTVRS FHHEEHLENI PGTSESSAFR FLFNLSSIPE
NEVISSAELR LFREQVDQGP DWEQGFHRIN IYEVMKPPAE MVPGHLITRL
LDTRLVHHNV TRWETFDVSP AVLRWTREKQ PNYGLAIEVT HLHQTRTHQG
QHVRISRSLP QGSGDWAQLR PLLVTFGHDG RGHTLTRRRA KRSPKHHPQR
SRKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN
STNHAIVQTL VNSVNSSIPK ACCVPTELSA ISMLYLDEYD KVVLKNYQEM
VVEGCGCR
UCSC uc007thd.2; mouse.;

Category

Mouse Bmp4 ELISA Kit

Mouse Bmp-4 ELISA Kit;Mouse bone morphogenetic protein 2B ELISA Kit;Mouse BMP-2B ELISA Kit;Mouse Dvr-4 ELISA Kit;Mouse Bmp2b ELISA Kit;Mouse Bmp2b-1 ELISA Kit;Mouse Bmp2b1 ELISA Kit;Mouse bone morphogenetic protein 4 ELISA Kit;