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Human GAPDH ELISA Kit

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  • Alternative name

    Human GAPDH ELISA Kit;Human peptidyl-cysteine S-nitrosylase GAPDH ELISA Kit;Human GAPD ELISA Kit;Human G3PD ELISA Kit;Human HEL-S-162eP ELISA Kit;Human glyceraldehyde-3-phosphate dehydrogenase ELISA Kit;Human aging-associated gene 9 protein ELISA Kit;Human epididymis secretory sperm binding protein Li 162eP ELISA Kit;
  • Catalog
    E016979
  • species
    Human
  • GeneGAPDH
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). No significant cross-reactivity or interference between Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.63ng/m.
  • Intended UseHuman GAPDH ELISA Kit allows for the in vitro quantitative determination of GAPDH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Glyceraldehyde-3-phosphate dehydrogenase Protein information
  • Uniprot ID G3P_HUMAN
  • Uniprot AC P04406; E7EUT4; P00354; Q53X65;
  • UniGene Hs.544577; Hs.592355; Hs.598320;
  • GeneID 2597
  • KEGG hsa:2597;
  • Human Glyceraldehyde-3-phosphate dehydrogenase Protein SEQUENCE

  • SEQUENCE 335 AA; 36053 MW; C9C135E8AE3E8744 CRC64;

    MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD

    STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG

    VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA

    SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR

    GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE

    KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG

    IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

  • UCSC uc031qfw.3; human. [P04406-1];



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