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Human MYH3 ELISA Kit

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  • Alternative name

    Human Muscle embryonic myosin heavy chain ELISA Kit;Human myosin heavy chain 3 ELISA Kit;Human myosin heavy chain, fast skeletal muscle, embryonic ELISA Kit;Human SMHCE ELISA Kit;Human DA2A ELISA Kit;Human DA2B ELISA Kit;Human DA8 ELISA Kit;Human HEMHC ELISA Kit;Human MYHC-EMB ELISA Kit;Human MYHSE1 ELISA Kit;Human myosin-3 ELISA Kit;Human myosin, heavy chain 3, skeletal muscle, embryonic ELISA Kit;Human myosin, heavy polypeptide 3, skeletal muscle, embryonic ELISA Kit;Human myosin, skeletal, heavy chain, embryonic 1 ELISA Kit;
  • Catalog
    E025427
  • species
    Human
  • GeneMYH3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). No significant cross-reactivity or interference between Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.126ng/mL.
  • Intended UseHuman MYH3 ELISA Kit allows for the in vitro quantitative determination of MYH3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Myosin-3 Protein information
  • Uniprot ID MYH3_HUMAN
  • Uniprot AC P11055; Q15492;
  • UniGene Hs.440895;
  • GeneID 4621
  • KEGG hsa:4621;
  • Human Myosin-3 Protein SEQUENCE

  • SEQUENCE 1940 AA; 223905 MW; B7D6AF219E88E5C8 CRC64;

    MSSDTEMEVF GIAAPFLRKS EKERIEAQNQ PFDAKTYCFV VDSKEEYAKG

    KIKSSQDGKV TVETEDNRTL VVKPEDVYAM NPPKFDRIED MAMLTHLNEP

    AVLYNLKDRY TSWMIYTYSG LFCVTVNPYK WLPVYNPEVV EGYRGKKRQE

    APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA

    ATGDLAKKKD SKMKGTLEDQ IISANPLLEA FGNAKTVRND NSSRFGKFIR

    IHFGTTGKLA SADIETYLLE KSRVTFQLKA ERSYHIFYQI LSNKKPELIE

    LLLITTNPYD YPFISQGEIL VASIDDAEEL LATDSAIDIL GFTPEEKSGL

    YKLTGAVMHY GNMKFKQKQR EEQAEPDGTE VADKTAYLMG LNSSDLLKAL

    CFPRVKVGNE YVTKGQTVDQ VHHAVNALSK SVYEKLFLWM VTRINQQLDT

    KLPRQHFIGV LDIAGFEIFE YNSLEQLCIN FTNEKLQQFF NHHMFVLEQE

    EYKKEGIEWT FIDFGMDLAA CIELIEKPMG IFSILEEECM FPKATDTSFK

    NKLYDQHLGK SNNFQKPKVV KGRAEAHFSL IHYAGTVDYS VSGWLEKNKD

    PLNETVVGLY QKSSNRLLAH LYATFATADA DSGKKKVAKK KGSSFQTVSA

    LFRENLNKLM SNLRTTHPHF VRCIIPNETK TPGAMEHSLV LHQLRCNGVL

    EGIRICRKGF PNRILYGDFK QRYRVLNASA IPEGQFIDSK KACEKLLASI

    DIDHTQYKFG HTKVFFKAGL LGTLEEMRDD RLAKLITRTQ AVCRGFLMRV

    EFQKMVQRRE SIFCIQYNIR SFMNVKHWPW MKLFFKIKPL LKSAETEKEM

    ATMKEEFQKT KDELAKSEAK RKELEEKLVT LVQEKNDLQL QVQAESENLL

    DAEERCDQLI KAKFQLEAKI KEVTERAEDE EEINAELTAK KRKLEDECSE

    LKKDIDDLEL TLAKVEKEKH ATENKVKNLT EELSGLDETI AKLTREKKAL

    QEAHQQALDD LQAEEDKVNS LNKTKSKLEQ QVEDLESSLE QEKKLRVDLE

    RNKRKLEGDL KLAQESILDL ENDKQQLDER LKKKDFEYCQ LQSKVEDEQT

    LGLQFQKKIK ELQARIEELE EEIEAERATR AKTEKQRSDY ARELEELSER

    LEEAGGVTST QIELNKKREA EFLKLRRDLE EATLQHEAMV AALRKKHADS

    VAELGEQIDN LQRVKQKLEK EKSEFKLEID DLSSSMESVS KSKANLEKIC

    RTLEDQLSEA RGKNEEIQRS LSELTTQKSR LQTEAGELSR QLEEKESIVS

    QLSRSKQAFT QQTEELKRQL EEENKAKNAL AHALQSSRHD CDLLREQYEE

    EQEGKAELQR ALSKANSEVA QWRTKYETDA IQRTEELEEA KKKLAQRLQD

    SEEQVEAVNA KCASLEKTKQ RLQGEVEDLM VDVERANSLA AALDKKQRNF

    DKVLAEWKTK CEESQAELEA SLKESRSLST ELFKLKNAYE EALDQLETVK

    RENKNLEQEI ADLTEQIAEN GKTIHELEKS RKQIELEKAD IQLALEEAEA

    ALEHEEAKIL RIQLELTQVK SEIDRKIAEK DEEIEQLKRN YQRTVETMQS

    ALDAEVRSRN EAIRLKKKME GDLNEIEIQL SHANRQAAET LKHLRSVQGQ

    LKDTQLHLDD ALRGQEDLKE QLAIVERRAN LLQAEVEELR ATLEQTERAR

    KLAEQELLDS NERVQLLHTQ NTSLIHTKKK LETDLMQLQS EVEDASRDAR

    NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNLEQT VKDLQHRLDE

    AEQLALKGGK KQIQKLETRI RELEFELEGE QKKNTESVKG LRKYERRVKE

    LTYQSEEDRK NVLRLQDLVD KLQVKVKSYK RQAEEADEQA NAHLTKFRKA

    QHELEEAEER ADIAESQVNK LRAKTRDFTS SRMVVHESEE

  • UCSC uc002gmq.3; human.;



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