Human PSAP ELISA Kit

Alternative name
Human proactivator polypeptide ELISA Kit;Human Protein A ELISA Kit;Human Cerebroside sulfate activator ELISA Kit;Human CSAct ELISA Kit;Human Dispersin ELISA Kit;Human Sphingolipid activator protein 1 ELISA Kit;Human SAP-1 ELISA Kit;Human Sulfatide/GM1 activator ELISA Kit;Human A1 activator ELISA Kit;Human Co-beta-glucosidase ELISA Kit;Human Glucosylceramidase activator ELISA Kit;Human Sphingolipid activator protein 2 ELISA Kit;Human SAP-2 ELISA Kit;Human Component C ELISA Kit;Human Protein C ELISA Kit;Human GLBA ELISA Kit;Human SAP1 ELISA Kit;Human prosaposin ELISA Kit;Human sphingolipid activator protein-1 ELISA Kit;
Catalog
E031219
species
Human
GenePSAP
SpecificityThis assay has high sensitivity and excellent specificity for detection of PSAP. No significant cross-reactivity or interference between PSAP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PSAP and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman PSAP ELISA Kit allows for the in vitro quantitative determination of PSAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: PSAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PSAP antibody and an PSAP-HRP conjugate. The assay sample and buffer are incubated together with PSAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSAP concentration since PSAP from samples and PSAP-HRP conjugate compete for the anti-PSAP antibody binding site. Since the number of sites is limited, as more sites are occupied by PSAP from the sample, fewer sites are left to bind PSAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSAP concentration in each sample is interpolated from this standard curve.
Human Saposin-D Protein information
Uniprot ID SAP_HUMAN
Uniprot AC PSAHKPLL GTEKCIWGPS YWCQNTETAA QCNAVEHCKR HVWN
UniGene Hs.523004;
GeneID 5660
KEGG hsa:5660;
Human Saposin-D Protein SEQUENCE
SEQUENCE 524 AA; 58113 MW; 71977F7A8C9E1533 CRC64;
MYALFLLASL LGAALAGPVL GLKECTRGSA VWCQNVKTAS DCGAVKHCLQ
TVWNKPTVKS LPCDICKDVV TAAGDMLKDN ATEEEILVYL EKTCDWLPKP
NMSASCKEIV DSYLPVILDI IKGEMSRPGE VCSALNLCES LQKHLAELNH
QKQLESNKIP ELDMTEVVAP FMANIPLLLY PQDGPRSKPQ PKDNGDVCQD
CIQMVTDIQT AVRTNSTFVQ ALVEHVKEEC DRLGPGMADI CKNYISQYSE
IAIQMMMHMQ PKEICALVGF CDEVKEMPMQ TLVPAKVASK NVIPALELVE
PIKKHEVPAK SDVYCEVCEF LVKEVTKLID NNKTEKEILD AFDKMCSKLP
KSLSEECQEV VDTYGSSILS ILLEEVSPEL VCSMLHLCSG TRLPALTVHV
TQPKDGGFCE VCKKLVGYLD RNLEKNSTKQ EILAALEKGC SFLPDPYQKQ
CDQFVAEYEP VLIEILVEVM DPSFVCLKIG ACPSAHKPLL GTEKCIWGPS
YWCQNTETAA QCNAVEHCKR HVWN
UCSC uc001jsm.4; human. [P07602-1];

Category

Human PSAP ELISA Kit