Human LGALS9 ELISA Kit

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  • Alternative name

    Human gal-9 ELISA Kit;Human ecalectin ELISA Kit;Human tumor antigen HOM-HD-21 ELISA Kit;Human HUAT ELISA Kit;Human LGALS9A ELISA Kit;Human galectin 9 ELISA Kit;Human galectin-9 ELISA Kit;Human lectin, galactoside-binding, soluble, 9 ELISA Kit;Human urate transporter/channel protein ELISA Kit;

  • Catalog
    E016023
  • species
    Human
  • GeneLGALS9
  • SpecificityNatural and recombinant human Galectin-9
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman LGALS9 ELISA Kit allows for the in vitro quantitative determination of LGALS9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human Galectin-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-9 has been precoated onto 96-well plates. Standards(E.coli, M1-T355) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-9 amount of sample captured in plate. Background: Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide.
  • Human Galectin-9 Protein information
  • Uniprot ID LEG9_HUMAN
  • Uniprot AC Q9NQ58;
  • UniGene Hs.81337;
  • GeneID 3965
  • KEGG hsa:3965;
  • Human Galectin-9 Protein SEQUENCE
  • SEQUENCE 355 AA; 39518 MW; 4748C22FCAFA536A CRC64;

    MAFSGSQAPY LSPAVPFSGT IQGGLQDGLQ ITVNGTVLSS SGTRFAVNFQ

    TGFSGNDIAF HFNPRFEDGG YVVCNTRQNG SWGPEERKTH MPFQKGMPFD

    LCFLVQSSDF KVMVNGILFV QYFHRVPFHR VDTISVNGSV QLSYISFQNP

    RTVPVQPAFS TVPFSQPVCF PPRPRGRRQK PPGVWPANPA PITQTVIHTV

    QSAPGQMFST PAIPPMMYPH PAYPMPFITT ILGGLYPSKS ILLSGTVLPS

    AQRFHINLCS GNHIAFHLNP RFDENAVVRN TQIDNSWGSE ERSLPRKMPF

    VRGQSFSVWI LCEAHCLKVA VDGQHLFEYY HRLRNLPTIN RLEVGGDIQL

    THVQT

  • UCSC uc060cvd.1; human.;



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