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Human GLI1 ELISA Kit

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  • Alternative name

    Human Glioma-associated oncogene ELISA Kit;Human oncogene GLI ELISA Kit;Human GLI ELISA Kit;Human GLI family zinc finger 1 ELISA Kit;Human zinc finger protein GLI1 ELISA Kit;Human GLI-Kruppel family member GLI1 ELISA Kit;Human glioma-associated oncogene 1 ELISA Kit;Human glioma-associated oncogene homolog 1 (zinc finger protein) ELISA Kit;
  • Catalog
    E016439
  • species
    Human
  • GeneGLI1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human GLI1. No significant cross-reactivity or interference between human GLI1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human GLI1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman GLI1 ELISA Kit allows for the in vitro quantitative determination of GLI1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLI1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Zinc finger protein GLI1 Protein information
  • Uniprot ID GLI1_HUMAN
  • Uniprot AC P08151; D0EUY3; E9PQQ9; F5H6H8; Q8TDN9;
  • UniGene Hs.632702;
  • GeneID 2735
  • KEGG hsa:2735;
  • Human Zinc finger protein GLI1 Protein SEQUENCE

  • SEQUENCE 1106 AA; 117904 MW; E29B11D1A6CD3D91 CRC64;

    MFNSMTPPPI SSYGEPCCLR PLPSQGAPSV GTEGLSGPPF CHQANLMSGP

    HSYGPARETN SCTEGPLFSS PRSAVKLTKK RALSISPLSD ASLDLQTVIR

    TSPSSLVAFI NSRCTSPGGS YGHLSIGTMS PSLGFPAQMN HQKGPSPSFG

    VQPCGPHDSA RGGMIPHPQS RGPFPTCQLK SELDMLVGKC REEPLEGDMS

    SPNSTGIQDP LLGMLDGRED LEREEKREPE SVYETDCRWD GCSQEFDSQE

    QLVHHINSEH IHGERKEFVC HWGGCSRELR PFKAQYMLVV HMRRHTGEKP

    HKCTFEGCRK SYSRLENLKT HLRSHTGEKP YMCEHEGCSK AFSNASDRAK

    HQNRTHSNEK PYVCKLPGCT KRYTDPSSLR KHVKTVHGPD AHVTKRHRGD

    GPLPRAPSIS TVEPKREREG GPIREESRLT VPEGAMKPQP SPGAQSSCSS

    DHSPAGSAAN TDSGVEMTGN AGGSTEDLSS LDEGPCIAGT GLSTLRRLEN

    LRLDQLHQLR PIGTRGLKLP SLSHTGTTVS RRVGPPVSLE RRSSSSSSIS

    SAYTVSRRSS LASPFPPGSP PENGASSLPG LMPAQHYLLR ARYASARGGG

    TSPTAASSLD RIGGLPMPPW RSRAEYPGYN PNAGVTRRAS DPAQAADRPA

    PARVQRFKSL GCVHTPPTVA GGGQNFDPYL PTSVYSPQPP SITENAAMDA

    RGLQEEPEVG TSMVGSGLNP YMDFPPTDTL GYGGPEGAAA EPYGARGPGS

    LPLGPGPPTN YGPNPCPQQA SYPDPTQETW GEFPSHSGLY PGPKALGGTY

    SQCPRLEHYG QVQVKPEQGC PVGSDSTGLA PCLNAHPSEG PPHPQPLFSH

    YPQPSPPQYL QSGPYTQPPP DYLPSEPRPC LDFDSPTHST GQLKAQLVCN

    YVQSQQELLW EGGGREDAPA QEPSYQSPKF LGGSQVSPSR AKAPVNTYGP

    GFGPNLPNHK SGSYPTPSPC HENFVVGANR ASHRAAAPPR LLPPLPTCYG

    PLKVGGTNPS CGHPEVGRLG GGPALYPPPE GQVCNPLDSL DLDNTQLDFV

    AILDEPQGLS PPPSHDQRGS SGHTPPPSGP PNMAVGNMSV LLRSLPGETE

    FLNSSA

  • UCSC uc001snx.4; human. [P08151-1];



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