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Human GCLC ELISA Kit

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  • Alternative name

    Human GCS heavy chain ELISA Kit;Human gamma-ECS ELISA Kit;Human gamma-glutamylcysteine synthetase ELISA Kit;Human GLCL ELISA Kit;Human GLCLC ELISA Kit;Human GCL ELISA Kit;Human GCS ELISA Kit;Human glutamate-cysteine ligase catalytic subunit ELISA Kit;Human glutamate--cysteine ligase catalytic subunit ELISA Kit;
  • Catalog
    E016798
  • species
    Human
  • GeneGCLC
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human GCLC. No significant cross-reactivity or interference between human GCLC and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human GCLC is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman GCLC ELISA Kit allows for the in vitro quantitative determination of GCLC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GCLC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GCLC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GCLC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GCLC bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Glutamate--cysteine ligase catalytic subunit Protein information
  • Uniprot ID GSH1_HUMAN
  • Uniprot AC P48506; Q14399;
  • UniGene Hs.654465;
  • GeneID 2729
  • KEGG hsa:2729;
  • Human Glutamate--cysteine ligase catalytic subunit Protein SEQUENCE

  • SEQUENCE 637 AA; 72766 MW; 511F33F106A15504 CRC64;

    MGLLSQGSPL SWEETKRHAD HVRRHGILQF LHIYHAVKDR HKDVLKWGDE

    VEYMLVSFDH ENKKVRLVLS GEKVLETLQE KGERTNPNHP TLWRPEYGSY

    MIEGTPGQPY GGTMSEFNTV EANMRKRRKE ATSILEENQA LCTITSFPRL

    GCPGFTLPEV KPNPVEGGAS KSLFFPDEAI NKHPRFSTLT RNIRHRRGEK

    VVINVPIFKD KNTPSPFIET FTEDDEASRA SKPDHIYMDA MGFGMGNCCL

    QVTFQACSIS EARYLYDQLA TICPIVMALS AASPFYRGYV SDIDCRWGVI

    SASVDDRTRE ERGLEPLKNN NYRISKSRYD SIDSYLSKCG EKYNDIDLTI

    DKEIYEQLLQ EGIDHLLAQH VAHLFIRDPL TLFEEKIHLD DANESDHFEN

    IQSTNWQTMR FKPPPPNSDI GWRVEFRPME VQLTDFENSA YVVFVVLLTR

    VILSYKLDFL IPLSKVDENM KVAQKRDAVL QGMFYFRKDI CKGGNAVVDG

    CGKAQNSTEL AAEEYTLMSI DTIINGKEGV FPGLIPILNS YLENMEVDVD

    TRCSILNYLK LIKKRASGEL MTVARWMREF IANHPDYKQD SVITDEMNYS

    LILKCNQIAN ELCECPELLG SAFRKVKYSG SKTDSSN

  • UCSC uc003pbx.5; human.;



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