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Human GFPT2 ELISA Kit

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  • Alternative name

    Human D-fructose-6-phosphate amidotransferase 2 ELISA Kit;Human glutamine:fructose-6-phosphate amidotransferase 2 ELISA Kit;Human GFAT 2 ELISA Kit;Human GFAT2 ELISA Kit;Human hexosephosphate aminotransferase 2 ELISA Kit;Human GFAT ELISA Kit;Human glutamine-fructose-6-phosphate transaminase 2 ELISA Kit;Human glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 ELISA Kit;Human glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 ELISA Kit;Human glutamine: fructose-6-phosphate aminotransferase 2 ELISA Kit;
  • Catalog
    E016829
  • species
    Human
  • GeneGFPT2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GFPT2. No significant cross-reactivity or interference between GFPT2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human GFPT2 is typically less than 0.114ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
  • Intended UseHuman GFPT2 ELISA Kit allows for the in vitro quantitative determination of GFPT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GFPT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GFPT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GFPT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GFPT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 Protein information
  • Uniprot ID GFPT2_HUMAN
  • Uniprot AC O94808; Q53XM2; Q9BWS4;
  • UniGene Hs.696497;
  • GeneID 9945
  • KEGG hsa:9945;
  • Human Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 Protein SEQUENCE

  • SEQUENCE 682 AA; 76931 MW; A090D75526F3192B CRC64;

    MCGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK

    ERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS

    AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA

    KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRR

    GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHA

    VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS

    DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN

    TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE

    LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGVTNT

    VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL

    QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS LLVMGRGYNY

    ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF

    AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS

    VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE

  • UCSC uc003mlw.2; human.;



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